![]() ![]() To date, rational design of gas-fermenting bacteria such as changing the expression levels of individual enzymes to obtain the desired pathway flux is challenging, because pathway design must follow a verifiable metabolic blueprint indicating where interventions should be executed. It will allow the microbial chassis to renewably valorize natural resources from carbon oxides, hydrogen, and/or lignocellulosic feedstocks more efficiently. Rational engineering of gas-fermenting bacteria for high yields of bioproducts is vital for a sustainable bioeconomy. Here we describe the database characteristics and implementation and demonstrate its use. The eQuilibrator code is open-source and all thermodynamic source data are freely downloadable in standardįormats. () enables easy calculation of Gibbs energies of compounds and reactions given arbitrary pH, ionic strength and metaboliteĬoncentrations. To address this problem, eQuilibrator couples a comprehensive and accurate database of thermodynamic properties of biochemicalĬompounds and reactions with a simple and powerful online search and calculation interface. ‘how much Gibbs energy is released by ATP hydrolysis at pH 5?’ are complicated excessively by the search for accurate data. However, thermodynamic data on biochemical compoundsĬan be difficult to find and is cumbersome to perform calculations with manually. That's the Way That the World Goes Round (9/22/01)Ħ.The laws of thermodynamics constrain the action of biochemical systems. I've got Todd covering Stones songs and Billy Joe Shaver compilations around here somewhere Great Day Coming > Amazing Grace > Amazing Grace It's the End of the World as We Know It > Oldest Todd show I know of from the Daily Planet, December 12, 1992 "In 1994, before moving to Idaho, I lived with Ryan at 607 E. I met Ryan in 1993 at the Fallout Shelter. Soon he was playing drums (temporarily) in my band Spawn. Ryan and I goofed around with a 4 track, recording many songs under the band name "Space Madness." This was with Thompson King on bass. We played one show as Space Madness, an outdoor concert in the parking lot behind Sadlack's Deli. Ryan and I would switch back and forth between vox/gtr and drums. ![]() I think it was around March 1994 that Thompson moved to Colorado, so Ryan and I started jamming with our friend Caspar Lee on drums. Ryan called it "the skylarks" 'cuz that's the car that his girlfriend drove. Don't ask me about her, I just want to set the record straight about the bands and songs. Skylarks played a couple of shows, including one at the Brewery. In between all this Ryan recorded a few solo songs on my 4 track. ![]() I still have all the masters from this era. Skylarks (Ryan: gtr/vox, Tristan: bass, Caspar: drums): Space Madness (Ryan: gtr/vox, Thompson: bass, Tristan: drums): They have not been officially released but it's been an idea for years here at Fort Hazel. He is certainly the most amazing and prolific songwriter I've ever (eek!) I've seen Ryan a few times since then, and in fact he's recorded a few songs at my studio in Boise: The other songs you have listed under "Space Madness Demos" were sung by me and I'm not particularly proud of my singing from that era. The track on HERE is called Poor Southern Girl, although it sounds like he could just as easily be singing porcelain I have no idea how that stuff got anywhere beyond my studio. This is the *lowest* fidelity recording I have of Ryan, and it was just him on gtr and vox. The Skylarks recordings were done 5/2/94 and include only 4 songs: public static, lucky star, texas, death on the road. Today, I run Fort Hazel, which is a 24track recording studio and really small indie label. Also I play keyboards in a cartoon jazz band called Lowbelly. I would love to put out the early Ryan stuff, but I want to do it right. ![]()
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